题 目：Decoding the core molecular machinery of autophagy

主 讲：Nobuo N. Noda 教授（北海道大学遗传医学研究所）

时 间：2025年11月8日(周六) 上午10:00-11:00

地 点：琳恩图书馆111报告厅

摘要：

Autophagy is a major degradative pathway in eukaryotic cells that maintains cellular homeostasis. Its hallmark is the de novo formation of the double-membrane organelle, the autophagosome; in principle, everything sequestered within an autophagosome is delivered to lysosomes for degradation. By integrating structural biology, in vitro reconstitution, and cell biological analyses, we are elucidating the molecular mechanisms of autophagosome biogenesis mainly using budding yeast as a model organism. In this talk, I will present our findings on how liquid–liquid phase separation builds the pre-autophagosomal structure (PAS) and on the mechanisms governing the initiation, expansion, and shaping of the isolation membrane, the precursor of the autophagosome. I would also like to mention the selective sequestration mechanism of protein condensates and mitochondria by autophagosomes, if time allows.

个人简介：

Professor Nobuo N. Noda is an outstanding scientist in the field of autophagy research who was mentored by Nobel laureate Professor Yoshinori Ohsumi. His remarkable contributions to cell biology, structural biology, and biochemistry have greatly advanced our understanding of the mechanisms of autophagy and intracellular membrane dynamics. Professor Noda primarily employs methods such as structural biology, biomechanics, and biochemistry to elucidate the structures of key autophagy protein complexes, reveal the molecular mechanisms of membrane morphological changes during autophagy, and uncover lipid transfer mechanisms. His notable work includes the identification of the crucial role of phase separation in the Atg1 complex in promoting autophagy initiation, the discovery of the lipid transfer function of the autophagy protein ATG2, and the lipid flipping function of ATG9. He has proposed a novel model for lipid transport during autophagosome growth. These findings have been published in high-impact journals such as Nature, Molecular Cell, and Nature Structural & Molecular Biology. His research achievements have not only garnered widespread attention in the academic community but have also provided important theoretical support and practical guidance for biomedical research.