时 间：2025年9月17日(周三) 下午14:00-15:00

地 点：琳恩图书馆111报告厅

主 讲：Ben Goult 教授（利物浦大学）

题 目：Mechanical Control of Enzymatic Processes via Talin

摘要：

Talin, the primary link between the ECM-binding integrin receptors and the actin cytoskeleton, coordinates the binding of a wide range of cytoskeletal and signaling adaptors in a force-dependent manner. Talin serves as a mechanosensitive signaling hub as its 13 force-dependent binary switch domains convert mechanical signals into biological signals by spatially localizing numerous signaling proteins in space and time to control signaling outputs. I will discuss how the mechanical signaling through talin represents a highly sophisticated machinery for controlling enzymatic processes.

The talk will focus on the enzymes that interact directly with talin, including CDK1 and PKA, as well as presenting new unpublished data on CDKL5, Src and ATAT-1 binding directly to talin, as each serves as a paradigm for a different mechanism for how mechanical signals can alter enzymatic signaling. I will discuss how understanding the different modes of scaffolding of each of these enzymes has enabled us to establish design principles of mechanically operated enzymes. 

Talin in conjunction with vinculin forms a meshwork of these switches, to form a meshwork code, or more concisely, a MeshCODE and the multiple switches within the talin rod function to process and store time- and force-dependent mechanical and chemical information. Most strikingly, the changing shape of a talin molecule as its pattern of switches unfold and refold introduces a spatial rearrangement of molecules within integrin adhesion sites, indicating a sophisticated mechanism for how alterations in the binary strings of talin molecules can lead to persistent changes in signaling and adhesion architecture.

个人简介：

Goult教授就职于英国利物浦大学（University of Liverpool），是国际知名的结构机械生物学家，其在细胞黏附、机械传感与记忆分子机制方面的研究具有开创性和广泛影响力。他的研究横跨结构生物学、生物物理、材料科学与神经科学，极具交叉性与前沿性，至今已发表论文92篇，h指数44，总引用超过6800次。