题 目：Capturing the native structure of membrane protein using vesicles（使用囊泡获取膜蛋白的天然结构）

主 讲：党尚宇 助理教授

主 持：廖茂富 讲席教授

时 间：2025年1月14日（星期二）上午10:00-11:30

地 点：琳恩图书馆111报告厅

报告摘要：

High-resolution structures of membrane proteins not only enhance our understanding of their molecular mechanisms but also facilitate drug development. However, structural studies of membrane proteins primarily rely on solubilization by detergents, which may not reflect their native states in a cellular context. In this talk, I will focus on our recent method that enables the structural determination of membrane proteins in their native lipid environment using vesicles. To tackle challenges during data processing, we also developed a micrograph-based sorting method for precise particle targeting, ultimately allowing us to achieve high-resolution structures. This method represents a promising and straightforward approach for studying the structure and function of membrane proteins in their native environment, eliminating the need for detergent screening and protein purification.

个人简介：

Shangyu Dang received his Ph.D. from Tsinghua University in 2014 and subsequently moved to the University of California, San Francisco, for postdoctoral training. He is now an Assistant Professor in the Division of Life Science at the Hong Kong University of Science and Technology. His research focuses on the molecular mechanisms of biological macromolecules, with particular interests in membrane proteins and protein-DNA complexes. His lab employs various approaches, including single-particle cryo-EM, cryo-ET, biochemistry, and electrophysiology. Additionally, his team is dedicated to developing new methods in the fields of single-particle cryo-EM and cryo-ET to address recurring challenges and expand their applications.