题目：A Mycovirus VIGS Vector Confers Hypovirulence to a Plant Pathogenic Fungus to Control Wheat FHB

主讲：郭立华 研究员（中国农业科学院植物保护研究所）

时间：11月2日（周四）11:20-12:20

会议地点：学习书坊（星巴克二楼）

报告摘要：Mycovirus-mediated hypovirulence has the potential to control fungal diseases. However, the availability of hypovirulence-conferring mycoviruses for plant fungal disease control is limited as most fungal viruses are asymptomatic. In this study, the virus-induced gene silencing (VIGS) vector p26-D4 of Fusarium graminearum gemytripvirus 1 (FgGMTV1), a tripartite circular single-stranded DNA mycovirus, is successfully constructed to convert the causal fungus of cereal Fusarium head blight (FHB) into a hypovirulent strain. p26-D4, with an insert of a 75-150 bp fragment of the target reporter transgene transcript in both sense and antisense orientations, efficiently triggered gene silencing in Fusarium graminearum. Notably, the two hypovirulent strains, p26-D4-Tri101, and p26-D4-FgPP1, obtained by silencing the virulence-related genes Tri101 and FgPP1 with p26-D4, can be used as biocontrol agents to protect wheat from a fungal disease FHB and mycotoxin contamination at the field level. This study not only describes the first mycovirus-derived VIGS system but also proves that the VIGS vector can be used to establish multiple hypovirulent strains to control pathogenic fungi.

个人简介：中国农业科学院植物保护研究所研究员，博士生导师。1994年本科毕业于内蒙古农业大学，1997年硕士毕业于内蒙古农业大学，2003年博士毕业于中国农业大学。聚焦生防真菌病毒资源的发掘与利用研究，包括真菌病毒的分子鉴定，真菌病毒、真菌与植物三者间互作的分子机制与协同进化研究，真菌病毒生物农药的研发。在Advanced Science, Science Advances, Nucleic Acids Research, Journal of Virology, Journal of Fungi等期刊发表论文40余篇，授权专利12项。获2020年中国农业科学院重大科学发现，中华农业科技二等奖1项、北京市科技二等奖2项、中国产学研合作创新成果奖一等奖1项，中国植物保护学会科学技术一等奖1项，中国农业科学院杰出科技创新奖1项。