Speaker: Prof. Shengjian JI, Prof. Chengzhi HU

Time: April 4, 2023 (Tuesday)

Venue: Room 111, Lynn Library

Life Sciences and Healthcare Engineering Interdisciplinary Forum

Topic: Application of microfluidic devices in neuroscience to study local translation in axons

Speaker: Prof. Shengjian JI (Associate Professor, School of Life Sciences)

Topic: Microrobotics and microsystems for single cell analysis

Speaker: Prof. Chengzhi HU (Associate Professor, Department of Mechanical and Energy Engineering)

Date: April 4, 2023 (Tuesday)

Time: 4:00-5:30 PM

Venue: Room 111, Lynn Library


姬生健博士毕业于北京大学,先后任美国约翰-霍普金斯大学医学院神经科学系博士后、康奈尔大学医学院研究助理教授。入选江苏特聘教授等重要人才计划。曾获得国家自然科学奖二等奖(一等奖空缺,第三完成人),教育部高等学校科学研究优秀成果奖自然科学一等奖(第四完成人)。多年来聚焦神经发育和功能的转录后调控机制研究,特别是在mRNA修饰和轴突内mRNA局部翻译等机制调控神经发育和功能等领域取得了一系列创新性研究成果,以通讯作者和第一作者(含共同)已在Neuron、Molecular Psychiatry、Nucleic Acids Research(2篇)、PLOS Biology、eLife、Advanced Science、Journal of Neuroscience、Cellular and Molecular Life Sciences 等国际主流期刊发表多篇论文。其课题组已经成为国际上研究RNA修饰调控神经发育和功能的最主要实验室(之一)。



Messenger RNA (mRNA) can be transported and targeted to different subcellular compartments and locally translated. Local translation is an evolutionally conserved mechanism that in mammals, provides an important tool to exquisitely regulate the subcellular proteome in different cell types, including neurons. Local translation in axons is involved in processes such as neuronal development, function, plasticity, and diseases. Microfluidic chambers are powerful tools for studying axonal mRNA localization and translation in neurons. In this talk, I will discuss our findings in characterizing the roles of axonally synthesized proteins, which either function locally in axons, or are retrogradely trafficked back to soma to achieve neuron-wide gene regulation. I will also highlight the development and application of new microfluidic devices in our lab to facilitate the local translation studies. 

Single-cell technology has proven to be a powerful tool for uncovering the cellular heterogeneity and functional diversity present in complex biological systems, providing insights into the behavior of cells in different conditions and environments. Microrobotics and microsystems, as miniaturized devices, facilitate high-throughput, cost-effective, and precise bioanalysis. Recently, we developed a label-free approach for intracellular extraction of organelles or molecules from living cells at subcellular resolution via a fully automated nanoprobing platform. Additionally, we have devised several multifunctional microelectrode and microfluidic devices to selectively form organoids, incorporating osteoprogenitor and vascular niches within a multiphasic scaffold. To further enhance targeted therapy, we have developed magnetic manipulation platforms and microrobots.

Next:Reimagined Proteomes To Precision Medicine Via Long Non-coding RNA.